关于举办“Preimplantation embryo-secreted factors modulate maternal gene expression in rat uterus”学术报告的通知
报告题目：Preimplantation embryo-secreted factors modulate maternal gene expression in rat uterus
报告人：Associate Professor Nobuhiko Yamauchi（日本九州大学山内伸彦副教授）
In mammalian reproduction, embryo implantation to the uterus spatiotemporally regulated by a complex process and which is triggered by a number of factors. Although the previous studies suggested that the uterine receptivity is mediated by blastocyst-derived factors, but specific functions of embryos remain to be defined during preimplantation. Therefore, the present study was conducted to identify the maternal genes regulated by embryo-secreted factors in the rat uterus. RNA sequencing (RNAseq) data revealed that 10 genes were up regulated in the delayed implantation uterus compared to the uterus of pseudopregnancy. The results from RNAseq were further verified by real time quantitative PCR and it was observed that Sulf1 expression was significantly (P<0.05) induced in the delayed implantation uterus, although Areg, Calca, Fxyd4, Lamc3 showed remarkable expression level but the expression level was not statistically significant. During early pregnancy, Areg, Calca, Fxyd4, Lamc3 and Sulf1 expression at 3.5 days post coitus (dpc) were significantly (P<0.05) higher than that of 1.5 dpc. Finally, embryo-conditioned media treatment revealed that Lamc3 and Sulf1 were up-regulated compared to other genes studied. These results suggest that embryo derived factors regulate the maternal gene expression and Lamc3 and Sulf1 can be the possible markers for response study to embryo-secreted factors, and thus further embryo-maternal communications will be possible to better understanding.
山内伸彦副教授，1995年毕业于日本东北大学，获博士学位。2003年至今任日本九州大学农学研究院副教授，现为九州大学动物生殖生理与生物技术实验室主任。山内伸彦副教授是日本著名动物生殖生理学专家，其研究主要集中于动物胚胎附植领域。截至目前，山内伸彦副教授共在Biology of Reproduction、Journal of Reproduction and Development、American Journal of Physiology-Cell Physiology、American Journal of Physiology-Endocrinology and Metabolism等生殖生物学和生理学领域著名术期刊发表研究论文80余篇。